Project Overview
The objective of this lab was to determine optimal conditions for the enzyme cellulase over time and to observe its operation under various conditions of temperature and pH. The hypothesis: The enzyme cellulase will optimally operate under conditions that are longer in duration, higher in temperature, and more basic. Lab groups performed an experiment that tested either rate of reaction, temperatures effect of cellulase activity, or pH’s effect on cellulase activity.
This experiment was outlined in “Laboratory Exercise #6 Determining the Optimal Conditions for Enzymatic Activity:” pages 47-56 found in Laboratory Manual for Biology104 (ed. Jacob, TC3 2014, accessed ANGEL on 10/08/14). Methodology for determining rate of reaction are described on pages 51-52. Upon calibrating the spectrometer20 at 555nm, a positive and negative control were created. 1.5ml of enzyme and 4.5ml of buffer (4.8pH) was added the positive tube while 1.5ml of dH20 and 4.5ml of buffer (4.8 pH) was added to the negative control tube. After placing the controls into a 50°C water bath, 1ml of each control was placed into a new positive/negative labeled tube at 15 minute intervals (1 hour total). Each sample was then tested for its glucose concentration using DNS assay (compared to a standard). Temperature and pH effect were determined by surrounding lab groups.
This experiment was outlined in “Laboratory Exercise #6 Determining the Optimal Conditions for Enzymatic Activity:” pages 47-56 found in Laboratory Manual for Biology104 (ed. Jacob, TC3 2014, accessed ANGEL on 10/08/14). Methodology for determining rate of reaction are described on pages 51-52. Upon calibrating the spectrometer20 at 555nm, a positive and negative control were created. 1.5ml of enzyme and 4.5ml of buffer (4.8pH) was added the positive tube while 1.5ml of dH20 and 4.5ml of buffer (4.8 pH) was added to the negative control tube. After placing the controls into a 50°C water bath, 1ml of each control was placed into a new positive/negative labeled tube at 15 minute intervals (1 hour total). Each sample was then tested for its glucose concentration using DNS assay (compared to a standard). Temperature and pH effect were determined by surrounding lab groups.